Linear and Circular Conformation of Pock-Forming Plasmid pSAl of Thiostrepton-Producing Streptomyces azureus ATCC 14921
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چکیده
منابع مشابه
Draft Genome Sequence of Thiostrepton-Producing Streptomyces azureus ATCC 14921
Streptomyces azureus ATCC 14921 belongs to the Streptomyces cyaneus cluster and is known to be a thiostrepton producer. Here, we report a draft genome sequence for this strain, consisting of 350 contigs containing a total of 8,790,525 bp, 8,164 predicted coding sequences, and a G+C content of 70.9%.
متن کاملMulticopy derivative of pock-forming plasmid pSA1 in Streptomyces azureus.
Streptomyces azureus carried one copy or less of plasmid pSA1, which elicited pocks at 0.1 to 1.0%. Strain PK100 was isolated from the wild-type strain after UV irradiation. PK100 carried approximately 20 to 30 copies of pSA1.1, a derivative of pSA1. Plasmid pSA1.1 elicited pocks at 100% and inhibited spore and thiostrepton production.
متن کاملIntroduction of Kanamycin Resistant Gene to Pock-forming Plasmid pSAl.1 of Streptomyces azureus and Identification of spolllE-like Gene in the Recombinant Plasmid
A plasmid, pSAl.1, of StrePtomyces a.zuyeus elicited conjugative pocks and inhibited the sporulation of its host mycelia. The analysis of plasmid pSA1.1 was hampered by the lack of selective marker. Therefore, the introduction of a kanamycin resistance gene (Fzmr) derived from Strefltomyces kanamyceticus to pSA1.1 was tried. The obtained recombinant still preserved the sporulation-inhibitory an...
متن کاملComplete Genome Sequence of Thiostrepton-Producing Streptomyces laurentii ATCC 31255
Streptomyces laurentii ATCC 31255 produces thiostrepton, a thiopeptide class antibiotic. Here, we report the complete genome sequence for this strain, which contains a total of 8,032,664 bp, 7,452 predicted coding sequences, and a G+C content of 72.3%.
متن کاملIsolation and characterization of a pock-forming plasmid pTA4001 from Streptomyces lavendulae.
A covalently closed circular (ccc) DNA with a size of 5.9 kb was isolated from a strain producing streptothricins , Streptomyces lavendulae No. 1080, and its restriction map was determined. Colonies of the plasmid-carrying strain formed pocks on the lawn of the plasmid-free derivatives of the same organism. The pock-forming ability of pTA4001 was confirmed by transformation of S. lividans with ...
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ژورنال
عنوان ژورنال: Journal of the Faculty of Agriculture, Kyushu University
سال: 1988
ISSN: 0023-6152
DOI: 10.5109/23882